✦ LIBER ✦

Protein Purification Protocols Volume 59 || The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds

✍ Scribed by Doonan, Shawn

Book ID: 120509054
Publisher: Humana Press
Year: 1996
Tongue: English
Weight: 949 KB
Edition: 1
Category: Article
ISBN-13: 9780896033368
DOI: 10.1385/0-89603-336-8:69

No coin nor oath required. For personal study only.

✦ Synopsis

A comprehensive collection of essential, time-tested recipes for successful protein fractionation and purification in any experimental circumstance. The protocols give step-by-step instructions on how to select a source for the protein of interest, how to obtain a usable initial extract, how to purify the protein from that extract using both chemical and molecular methods, and how to dry and store the purified protein. Protein Purification Protocols provides all that is needed to design and carry out a successful purification program. It helps both experienced and novice investigators to clarify and define their purification problems and then provides a comprehensive set of tools for a practical solution.

The book contains black-and-white illustrations.

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Metabisulfite, diethyldithiocarbamate, and thiourea are potent phenoloxidase inhibitors commonly used during the extraction of plant proteins. Their effects on several amino acid derivatives and peptides are reported. Important and pH-dependent changes are induced by metabisulfite in the uv-absorpti