Protein-primed replication of bacteriophage PRD1 genome in vitro
β Scribed by Seung-Ku Yoo; Junetsu Ito
- Book ID
- 113209137
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 547 KB
- Volume
- 170
- Category
- Article
- ISSN
- 0042-6822
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## Abstract Protein p6 of __B. subtilis__ bacteriophage Γ29 binds to DNA forming a nucleoprotein complex in which the DNA wraps a protein core forming a rightβhanded superhelix, therefore restraining positive supercoiling and compacting the DNA. The protein does not specifically recognize a nucleot
Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magn