Protein patterns of gel-entrapped Escherichia coli cells differ from those of free-floating organisms
✍ Scribed by Frederique Perrot; Michel Hébraud; Roland Charlionet; Guy-Alain Junter; Thierry Jouenne
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 400 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
The two-dimensional electrophoretic patterns of cellular proteins from gel-entrapped Escherichia coli cells were compared to those of exponential- and stationary-phase free-floating organisms. The amounts of several proteins in immobilized cells were significantly different from those in free bacteria. Immobilized organisms rapidly produced a high level of dipeptide permease and a single-strand binding protein, and progressively accumulated an aldehyde dehydrogenase. Immobilization also induced a decrease in the levels of two proteins, i.e., the YFID protein and a DNA-binding, stationary-phase protein. The possible role of these proteins in the high resistance of immobilized bacteria to stresses is discussed.