Protein Misfolding Diseases: Methods and Protocols

✍ Scribed by Cláudio M. Gomes

• Publisher: Springer New York,Humana Press
• Year: 2019
• Tongue: English
• Leaves: 338
• Series: Methods in Molecular Biology 1873
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed book gathers a broad collection of experimental approaches to assist researchers in setting up different methods to investigate protein conformational disorders. Beginning with a section on assays focusing on biophysical approaches to study protein (mis)folding, the volume continues with sections on cellular and proteostasis assays as well as assays for protein folding correction and recovery, combining methods such as thermal shift assays, in silico improvement of protein solubility, and compound screening, an important area of research as it may open avenues for therapeutic strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protein Misfolding Diseases: Methods and Protocols serves as an ideal guide for researchers seeking to advance our knowledge of protein conformational disorders.

✦ Table of Contents

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation (Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes)....Pages 3-18
Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates (Eri Saijo, Bradley R. Groveman, Allison Kraus, Michael Metrick, Christina D. Orrù, Andrew G. Hughson et al.)....Pages 19-37
Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides (Ravit Malishev, Sofiya Kolusheva, Raz Jelinek)....Pages 39-51
Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis (Naoto Soya, Ariel Roldan, Gergely L. Lukacs)....Pages 53-67
A Multipronged Method for Unveiling Subtle Structural–Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies (Donatella Bulone, Pier Luigi San Biagio, Tatiana Quiñones-Ruiz, Manuel Rosario-Alomar, Igor K. Lednev, Frank T. Robb et al.)....Pages 69-92
High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1 (Alireza Abdolvahabi, Sanaz Rasouli, Corbin M. Croom, Devon L. Plewman)....Pages 93-108
Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases (Devkee M. Vadukul, Youssra K. Al-Hilaly, Louise C. Serpell)....Pages 109-122
Assays for Light Chain Amyloidosis Formation and Cytotoxicity (Luis M. Blancas-Mejia, Pinaki Misra, Christopher J. Dick, Marta Marin-Argany, Keely R. Redhage, Shawna A. Cooper et al.)....Pages 123-153
Front Matter ....Pages 155-155
Monitoring Aggregate Clearance and Formation in Cell-Based Assays (Evelien Eenjes, Young Joo Yang-Klingler, Ai Yamamoto)....Pages 157-169
Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor (Yu Liu, Matthew Fares, Xin Zhang)....Pages 171-182
Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation (Vibha Prasad, Aaron Voigt)....Pages 183-193
Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry (Salvador Ventura, Susanna Navarro)....Pages 195-212
Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells (Edward Pokrishevsky, Jeremy Nan, Neil R. Cashman)....Pages 213-224
A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction (Cagla Cömert, Paula Fernandez-Guerra, Peter Bross)....Pages 225-239
Superresolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells (Steffen J. Sahl, Willianne I. M. Vonk)....Pages 241-251
Front Matter ....Pages 253-253
Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry (Tânia G. Lucas, Cláudio M. Gomes, Bárbara J. Henriques)....Pages 255-264
Methods to Screen Compounds Against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics (Giulia Diniz da Silva Ferretti, Danielly C. Ferraz da Costa, Jerson L. Silva, Luciana Pereira Rangel)....Pages 265-277
Early Stage Discovery and Validation of Pharmacological Chaperones for the Correction of Protein Misfolding Diseases (Oscar Aubi, Per M. Knappskog, Aurora Martinez)....Pages 279-292
Constructing Kinetically Controlled Denaturation Isotherms of Folded Proteins Using Denaturant-Pulse Chaperonin Binding (Pierce T. O’Neil, Alexandra J. Machen, Jackie A. Thompson, Wei Wang, Quyen Q. Hoang, Michael R. Baldwin et al.)....Pages 293-304
In Vitro Prion Amplification Methodology for Inhibitor Screening (Tuane Cristine R. G. Vieira, Jerson L. Silva)....Pages 305-316
SolubiS: Optimizing Protein Solubility by Minimal Point Mutations (Rob van der Kant, Joost van Durme, Frederic Rousseau, Joost Schymkowitz)....Pages 317-333
Back Matter ....Pages 335-338

✦ Subjects

Life Sciences; Protein Science