Protein-Ligand Interactions Volume 305 || Probing Heme Protein–Ligand Interactions by UV/Visible Absorption Spectroscopy

✍ Scribed by Nienhaus, G. Ulrich

• Book ID: 120603863
• Publisher: Humana Press
• Year: 2005
• Tongue: English
• Weight: 488 KB
• Category: Article
• ISBN-13: 9781592599127
• DOI: 10.1385/1-59259-912-5:215

✦ Synopsis

A Readily Reproducible Collection Of Established And Emerging Techniques For Studying The Interaction Between Proteins And Ligands, Including Biochemical/bulk Techniques, Structure Analysis, Spectroscopy, Single-molecule Studies, And Theoretical/computational Tools. Among The Highlights Are Surface Plasmon Resonance (spr) And Reflectometric Biosensor Approaches, High-throughput Screening With Confocal Optics Microscopy, Single Molecule Fluorescence And Fluorescence Correlation Spectroscopy (fcs), Atomic Force Microscopy (afm), Crystallography Of Reaction Intermediates, And Time-resolved X-ray Crystallography. The Protocols Follow The Successful Methods In Molecular Biologytm Series Format, Each Offering Step-by-step Laboratory Instructions, An Introduction Outlining The Principle Behind The Technique, Lists Of The Necessary Equipment And Reagents, And Tips On Troubleshooting And Avoiding Known Pitfalls.