Protein-Ligand Interactions: Methods and
β Edwin A. Lewis, Kenneth P. Murphy (auth.), G. Ulrich Nienhaus (eds.)
π Library
π
2005
π Humana Press
π English
β¦ LIBER β¦
π
Protein-ligand Interactions: Methods and Applications
β Scribed by G. Ulrich Nienhaus
- Publisher
- Humana Press
- Year
- 2010
- Tongue
- English
- Leaves
- 580
- Series
- Methods in Molecular Biology
- Edition
- 1st Edition.
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biologyβ’ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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