PROTEIN KINASE C AFFECTS REFORMATION OF ENDOTHELIAL JUNCTIONS IN XENOPUS XTH-2 CELLS

✍ Scribed by Nicole Susann Werner; Rainer Meyer; Friedhelm Achenbach

• Book ID: 102567535
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 449 KB
• Volume: 23
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1998.0318

✦ Synopsis

Endothelial cells can reversibly be forced to suppress the formation of endothelial junctions (EJ) by cultivation in a low calcium medium. The authors localized vinculin and cadherin as marker proteins of EJ and actin as a cytoskeletal component by fluorescence microscopy, and used this cell model to study the reformation of endothelial junctions under conditions of activation and inhibition of protein kinase C (PKC). Inhibition of PKC by H-7 leads to an acceleration of EJ reformation, while constitutive activation by TPA inhibits the reformation process.