Protein kinase activity in cell free extracts ofStreptomyces avermitilis; comparing wild type and overproducing mutant

✍ Scribed by I. Lichá; J. Janeček; Z. Vaněk

• Book ID: 104635412
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 369 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00134193

✦ Synopsis

Protein kinase activity was assayed in cell-free extracts prepared from mycelia of a wild strain (MA) and an overproducing mutant (RX 2) of Streptomyces avermitilis. At least 10 different polypeptides ranging in Mr 120 000 were from lo ooo3% found to be phosphorylated on analysis of labeled cell lysates by gel electrophoresis and phosphorimage. The protein profile and the level of phosphorylation varied depending on the culture stage of the mycelia.