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Protein Identification by MALDI-TOF-MS Peptide Mapping: A New Strategy

✍ Scribed by Egelhofer, Volker; Gobom, Johan; Seitz, Harald; Giavalisco, Patrick; Lehrach, Hans; Nordhoff, Eckhard

Book ID
126481231
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
712 KB
Volume
74
Category
Article
ISSN
0003-2700

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Alternating current (AC) has been employed to enhance the efficiency of chymotryptic proteolysis for peptide mapping. It was allowed to flow through the mixture solution of proteins and chymotrypsin via a pair of platinum wire electrodes. Bovine serum albumin (BSA) and cytochrome c (Cyt-c) were dige