Protein hydrolysis with immobilized enzymes
β Scribed by C. C. Detar; R. D. Mason; H. H. Weetall
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 103 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-3592
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A new method combining protein chemistry with enzymes immobilized to paramagnetic beads is presented. The immobilized enzymes can substitute for regular enzymes in a number of protein chemistry protocols, resulting in faster reaction times, less sample contamination, and improved interfacing to mode
The present work deals with maltodextrin hydrolysis by glucoamylase immobilized onto corn stover in a fluidized bed reactor. An industrial enzyme preparation was covalently grafted onto corn stover, yielding an activity of up to 372 U/g and 1700 U/g for support particle sizes of 0.8 and 0.2 mm, resp
## Abstract Haloalkane dehalogenase from __Rhodococcus rhodochrous__ was covalently immobilized onto a polyethyleneimine impregnated Ξ³βalumina support. The dehalogenating enzyme was found to retain greater than 40% of its original activity after immobilization, displaying an optimal loading (max. a