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Protein extracts for nutritional purposes from fragile strains of Saccharomyces cerevisiae: Construction of strains and conditions for lysis

✍ Scribed by Dr. Lubomira Stateva; Pencho Venkov; Nikola Lyutskanov; Lidia Koleva; Asen Hadjiolov

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
415 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

A superfragile strain. Saccharorn,vces cerevisiae 21 1, carrying three nonallelic nuclear determinants of cell lysis by osmotic shock was isolated from the haploid progeny ofacross between two laboratory fragile strains VY 1 I60 and SY 15. The strain Sacch. cerevisiae 21 1 is prototrophic. grows well in both laboratory and industrial media, only when supplemented with osmotic stabilizers. The average protein content of its biomass after growth in a molasses-based nutritional medium is 48.7':b. In laboratory conditions this strain releases about 60'1/, of its cellular proteins by spontaneous lysis in water. However, on a pilot scale the protein yield decreases to about 25'x. After a single disintegration step at 650 atm, practically all of the cellular protein -91.5%. is extracted without any additional chemical treatment. This result cannot be reached in any of the wild type strains tested even after triple treatment at 650 atm.

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