Protein Engineering Handbook, Volume 1 & Volume 2

• Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
• Year: 2009
• Tongue: English
• Leaves: 992
• Category: Library

✦ Synopsis

Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.

Content:
Chapter 1 Guidelines for the Functional Analysis of Engineered and Mutant Enzymes (pages 1–13): Dale E. Edmondson and Giovanni Gadda
Chapter 2 Engineering Enantioselectivity in Enzyme?Catalyzed Reactions (pages 15–46): Romas Kazlauskas
Chapter 3 Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes (pages 47–79): Stefanie Jonas and Florian Hollfelder
Chapter 4 ??Value Analysis of Protein Folding Transition States (pages 81–120): Neil Ferguson and Alan R. Fersht
Chapter 5 Protein Folding and Solubility: Pathways and High?Throughput Assays (pages 121–145): Adam C. Fisher, Thomas J. Mansell and Matthew P. DeLisa
Chapter 6 Protein Dynamics and the Evolution of Novel Protein Function (pages 147–185): Jorg Zimmermann, Megan C. Thielges, Wayne Yu and Floyd E. Romesberg
Chapter 7 Gaining Insight into Enzyme Function through Correlation with Protein Motions (pages 187–211): Nicolas Doucet and Joelle N. Pelletier
Chapter 8 Structural Frameworks Suitable for Engineering (pages 213–232): Birte Hocker
Chapter 9 Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space (pages 233–269): Ana Beloqui, Miren Zumarraga, Miguel Alcalde, Peter N. Golyshin and Manuel Ferrer
Chapter 10 Inteins in Protein Engineering (pages 271–293): Alison R. Gillies and David W. Wood
Chapter 11 From Prospecting to Product – Industrial Metagenomics Is Coming of Age (pages 295–323): Jurgen Eck, Esther Gabor, Klaus Liebeton, Guido Meurer and Frank Niehaus
Chapter 12 Computational Protein Design (pages 325–342): Jeffery G. Saven
Chapter 13 Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts (pages 343–362): Kevin K. Desai and Brian G. Miller
Chapter 14 Designing Programmable Protein Switches (pages 363–389): Martin Sagermann
Chapter 15 The Cyclization of Peptides and Proteins with Inteins (pages 391–407): Blaise R. Boles and Alexander R. Horswill
Chapter 16 A Method for Rapid Directed Evolution (pages 409–439): Manfred T. Reetz
Chapter 17 Evolution of Enantioselective Bacillus subtilis Lipase (pages 441–451): Thorsten Eggert, Susanne A. Funke, Jennifer N. Andexer, Manfred T. Reetz and Karl?Erich Jaeger
Chapter 18 Circular Permutation of Proteins (pages 453–471): Glenna E. Meister, Manu Kanwar and Marc Ostermeier
Chapter 19 Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): A Versatile Tool for Generating Targeted Libraries (pages 473–480): Asael Herman and Dan S. Tawfik
Chapter 20 Protein Engineering by Structure?Guided SCHEMA Recombination (pages 481–492): Gloria Saab?Rincon, Yougen Li, Michelle Meyer, Martina Carbone, Marco Landwehr and Frances H. Arnold
Chapter 21 Chimeragenesis in Protein Engineering (pages 493–514): Manuela Trani and Prof. Dr. Stefan Lutz
Chapter 22 Protein Generation Using a Reconstituted System (pages 515–535): Bei?Wen Ying and Takuya Ueda
Chapter 23 Equipping in vivo Selection Systems with Tunable Stringency (pages 537–561): Martin Neuenschwander, Andreas C. Kleeb, Peter Kast and Donald Hilvert
Chapter 24 Protein Engineering by Phage Display (pages 563–603): Agathe Urvoas, Philippe Minard and Patrice Soumillion
Chapter 25 Screening Methodologies for Glycosidic Bond Formation (pages 605–620): Amir Aharoni and Stephen G. Withers
Chapter 26 Yeast Surface Display in Protein Engineering and Analysis (pages 621–648): Benjamin J. Hackel and Dane Wittrup
Chapter 27 In Vitro Compartmentalization (IVC) and Other High?Throughput Screens of Enzyme Libraries (pages 649–667): Amir Aharoni and Dan S. Tawfik
Chapter 28 Colorimetric and Fluorescence?Based Screening (pages 669–711): Jean?Louis Reymond
Chapter 29 Confocal and Conventional Fluorescence?Based High Throughput Screening in Protein Engineering (pages 713–751): Ulrich Haupts, Oliver Hesse, Michael Strerath, Peter J. Walla and Wayne M. Coco
Chapter 30 Alteration of Substrate Specificity and Stereoselectivity of Lipases and Esterases (pages 753–775): Dominique Bottcher, Marlen Schmidt and Uwe T. Bornscheuer
Chapter 31 Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering (pages 777–796): Matthew DeSieno, Jing Du and Huimin Zhao
Chapter 32 Protein Engineering of Modular Polyketide Synthases (pages 797–827): Alice Y. Chen and Chaitan Khosla
Chapter 33 Cyanophycin Synthetases (pages 829–848): Anna Steinle and Alexander Steinbuchel
Chapter 34 Biosynthetic Pathway Engineering Strategies (pages 849–876): Claudia Schmidt?Dannert and Alexander Pisarchik
Chapter 35 Natural Polyester?Related Proteins: Structure, Function, Evolution and Engineering (pages 877–914): Seiichi Taguchi and Takeharu Tsuge
Chapter 36 Bioengineering of Sequence?Repetitive Polypeptides: Synthetic Routes to Protein?Based Materials of Novel Structure and Function (pages 915–938): Sonha C. Payne, Melissa Patterson and Vincent P. Conticello
Chapter 37 Silk Proteins – Biomaterials and Bioengineering (pages 939–959): Xiaoqin Wang, Peggy Cebe and David. L. Kaplan