✦ LIBER ✦

Protein diffusion coefficient measurements by laminar flow analysis: Method and applications

✍ Scribed by Rodney R. Walters; John F. Graham; Robert M. Moore; David J. Anderson

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 446 KB
Volume: 140
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90152-0

No coin nor oath required. For personal study only.

✦ Synopsis

Laminar flow in an open tube leads to solute band spreading. This process is opposed by radial diffusion. The translational diffusion coefficient of a solute can be calculated from the band dispersion, tube length, and flow rate. No calibration is necessary. A simple apparatus was used to measure the diffusion coefficients of several small solutes and 18 proteins. Most results were within +/- 10% of literature values. Each determination required 7-90 min, depending on the magnitude of the diffusion coefficient. The amount of protein needed was approximately 25 micrograms. The diffusion coefficient data were useful for the estimation of molecular weights.

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