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Protein Denaturation by Addition and Removal of Acetonitrile: Application to Tryptic Digestion of Acetylcholinesterase

โœ Scribed by R. Haas; T.L. Rosenberry


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
202 KB
Volume
224
Category
Article
ISSN
0003-2697

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By adding salts (sodium chloride, ammonium sulfate), it is possible to induce phase separation in membraneprotein solubilisates containing Triton \(\mathrm{X}-100\) or Nonidet \(\mathrm{P}-40\) at temperatures between 0 and \(20^{\circ} \mathrm{C}\). Other nonionic detergents of the Brij, Lubrol, an