Protein-coated poly(L-lactic acid) fibers provide a substrate for differentiation of human skeletal muscle cells

✍ Scribed by Cronin, Elizabeth M. ;Thurmond, Frederick A. ;Bassel-Duby, Rhonda ;Williams, R. Sanders ;Wright, Woodring E. ;Nelson, Kevin D. ;Garner, Harold R.

• Book ID: 102294301
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 258 KB
• Volume: 69A
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.a.30009

✦ Synopsis

Abstract

Tissue engineering represents a potential method for repairing damaged skeletal muscle tissue. Extracellular matrix (ECM) proteins were evaluated for their ability to aid in cell attachment, whereas a poly(L‐lactic acid) (PLLA) fiber scaffold was tested as a substrate for the differentiation of human skeletal muscle cells. In comparison to uncoated or gelatin‐coated PLLA films, cell attachment increased significantly (p < 0.001) on PLLA films coated with ECM gel, fibronectin, or laminin. Myoblasts differentiated into multinucleated myofibers on ECM gel‐coated PLLA fibers, and expressed muscle markers such as myosin and α‐actinin. Oligonucleotide microarray analysis showed similar gene expression profiles for human skeletal muscle cells on ECM gel‐coated PLLA fibers as to that observed for myofibers on tissue culture plates. Therefore, PLLA fibers coated with ECM proteins provide a scaffold for the development of skeletal muscle tissue for tissue engineering and cell transplantation applications. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 373–381, 2004