Protein chip technology

✍ Scribed by Heng Zhu; Michael Snyder

• Book ID: 104414618
• Publisher: Elsevier Science
• Year: 2003
• Tongue: English
• Weight: 604 KB
• Volume: 7
• Category: Article
• ISSN: 1367-5931
• DOI: 10.1016/s1367-5931(02)00005-4

✦ Synopsis

Microarray technology has become a crucial tool for large-scale and high-throughput biology. It allows fast, easy and parallel detection of thousands of addressable elements in a single experiment. In the past few years, protein microarray technology has shown its great potential in basic research, diagnostics and drug discovery. It has been applied to analyse antibody±antigen, protein±protein, protein±nucleic-acid, protein±lipid and protein±small-molecule interactions, as well as enzyme±substrate interactions. Recent progress in the ®eld of protein chips includes surface chemistry, capture molecule attachment, protein labeling and detection methods, high-throughput protein/antibody production, and applications to analyse entire proteomes.