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Protein Backbone 15N Relaxation Rates as a Tool for the Diagnosis of Structure Quality

✍ Scribed by Eva de Alba; Nico Tjandra

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 59 KB
Volume: 144
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.2000.2063

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✦ Synopsis

In the work reported herein we define a structure validation factor that depends on protein backbone 15 N relaxation rates. This is an alternative method to the previously defined quality factors derived from anisotropic chemical shifts or residual dipolar couplings. We have used the structure dependence of 15 N relaxation rates of anisotropically tumbling proteins to calculate this structure diagnosis factor and have used it to demonstrate the improvement of protein structures refined with residual dipolar couplings.

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