The characteristics of protein detection and quantitation with SYPRO Ruby protein gel stain in one- and two-dimensional polyacrylamide gels were evaluated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of three different purified recombinant proteins showed that the l
β¦ LIBER β¦
Protein analysis on two-dimensional polyacrylamide gels in the femtogram range: Use of a new sulfur-labeling reagent
β Scribed by Ysander V. Boxberg
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 918 KB
- Volume
- 169
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
An ultrasensitive staining procedure for two-dimensional polyacrylamide gels of protein homogenates has been developed. It combines the use of ultrathin gels and the labeling of proteins by a 35S-labeling reagent.
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