Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100
✦ LIBER ✦
Protein analysis by mass spectrometry and sequence database searching: A proteomic approach to identify human lymphoblastoid cell line proteins
✍ Scribed by Raymonde Joubert-Caron; Jean-Pierre Le Caër; Fréderic Montandon; Florence Poirier; Michel Pontet; Nïma Imam; Jean Feuillard; Dominique Bladier; Jean Rossier; Michel Caron
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 528 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0173-0835
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## Abstract The proteomic profiles of a human hepatoma revertant, CL1, and its original cell line, SMMC7721, were compared by using an improved two‐dimensional electrophoresis (2‐DE) procedure, with multi‐IPGstrips gels (length ≤ 13 cm) run simultaneously on one sodium dodecyl sulfate (SDS) gel (sh