Protein adsorption on crosslinked polydimethylsiloxane using total internal reflection fluorescence
β Scribed by Brent K Lok; Yu-Ling Cheng; Channing R Robertson
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 927 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0021-9797
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Spatially-resolved detection of antibody-antigen reactions at the solid/liquid interface was investigated by total internal reflection excited fluorescence from large area flat surfaces. Anti-HSA immunoglobulin G (IgG) antibody was immobilized at four spatially distinct spots. Binding of fluorescein
## Abstract We have used total internal reflection fluorescence (TIRF) to measure the adsorption kinetics of a newly synthesized fluorescent derivative of a triblock copolymer comprising two poly(ethylene oxide) arms connected by a poly(butylene oxide) segment. The composition is (EO)~400~ (BO)~55~