Protein adsorption in polysulfone hollow fiber bioreactors used for serum-free mammalian cell culture
✍ Scribed by Anant Y. Patkar; Bruce D. Bowen; James M. Piret
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 728 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
The recovery of serum-free medium proteins from polysulfone hollow fiber bioreactors (HFBRs) w a s investigated. More than 99% of the initial transferrin w a s adsorbed to the hydrophobic hollow fibers within 2 h of HFBR operation. A methodology to minimize transferrin adsorption by pre-adsorption of bovine serum albumin (BSA) w a s developed. BSA adsorption on suspended cut fibers w a s virtually complete within 1 h. BSA-coated fibers adsorbed only 5% of the transferrin within 10 days, whereas uncoated cut fibers adsorbed more than 99% of the transferrin within 1 h. An improved HFBR startup procedure, using a BSA-coating step before inoculation, resulted in substantially higher transferrin recovery. Additional factors influenced extracapillary space (ECS) transferrin concentrations. Pronounced downstream polarization of transferrin was observed in the ECS. In addition, the 30-kDa nominal molecular weight cutoff ultrafiltration membranes rapidly leaked transferrin from the ECS to the lumen.