Protective role of heme oxygenase-1 in oxidative stress-induced neuronal injury

✍ Scribed by Wei-dong Le; Wen-jie Xie; Stanley H. Appel

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 153 KB
• Volume: 56
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19990615)56:6<652::aid-jnr11>3.0.co;2-5

✦ Synopsis

Heme oxygenase-1 (HO-1) is a stress protein induced in response to a variety of oxidative challenges. After treatment of the hybrid septal cells SN 56 with ␤-amyloid peptide (␤-AP 1-40 ) or hydrogen peroxide (H 2 O 2 ), we detected high levels of reactive oxygen species, accompanied by a significant elevation in HO-1 expression. Levels of HO-1 increased and then decreased following cell loss. Pretreatment of SN 56 cells with HO-1 antisense oligonucleotides dramatically decreased the immunoreactivity of HO-1 and significantly enhanced the cytotoxicity of ␤-AP 1-40 and H 2 O 2 . In contrast, pretreatment with hemin, an HO-1 inducer, increased the expression of HO-1 and decreased the ␤-AP 1-40 -and H 2 O 2 -induced cytotoxicity. These findings support the importance of HO-1 in protecting neurons against oxidative stress-induced injury.