Protective factors against oxygen free radicals and hydrogen peroxide in rheumatoid arthritis synovial fluid

✍ Scribed by Pieter Biemond; Antonius J. G. Swaak; Prof. Johan F. Koster

• Book ID: 102751440
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 526 KB
• Volume: 27
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780270706

✦ Synopsis

Oxygen free radicals are probably involved in the pathogenesis of rheumatoid arthritis (RA). The enzymes involved in protection against oxygen free radicals and Hz02 (superoxide dismutase, catalase, and glutathione peroxidase) were measured. Superoxide dismutase was not increased, glutathione peroxidase was slightly and catalase was strongly elevated in RA synovial fluid (SF) compared with control SF. Although these enzymes are present in SF, the activities are insufficient to protect against oxygen free radicals and HzO2. In contrast to transferrin, ferritin was increased in RA synovial fluid. Ceruloplasmin was also elevated. When rat liver microsomes were used as a target for oxygen free radicals, serum and SF were both protective. Gel filtration experiments showed that the fraction pattern in which there was maximal protective potential against lipid peroxidation corresponded closely to the level of ceruloplasmin. After removal of ceruloplasmin from serum or SF, about 70% of the protective capacity disappeared. It is concluded that ceruloplasmin is an important protector against oxygen free radicals.

Evidence is accumulating which suggests that oxygen free radicals such as superoxide (027 and