Protective activities of ribosomal ribonucleic acid and lipopolysaccharide ofPseudomonas aeruginosa: a comparative study

✍ Scribed by R. Gonggrijp; W. J. H. A. Mullers; C. P. A. Boven

• Publisher: Springer Netherlands
• Year: 1983
• Tongue: English
• Weight: 570 KB
• Volume: 49
• Category: Article
• ISSN: 0003-6072
• DOI: 10.1007/bf00393676

✦ Synopsis

Ribosomal ribonucleic acid (RNA) and lipopolysaccharide (LPS) from P. aerug&osa were compared with respect to their protective activities in mice against an infection with P. aeruginosa. This study is concentrated on the protective activity of RNA. RNA isolated from purified ribosomes did not contain LPS as determined with the Limulus test. Injection of RNA with the adjuvant dimethyldioctadecylammonium bromide (DDA) protected mice against P. aeruginosa without inducing LPS-specific antibodies. C3H/HeJ mice which are relatively insensitive to the protective activity of LPS could be protected with RNA. The protective activities of RNA and LPS from a mutant strain ofP. aeruginosa, PAC 605, containing defective lipopolysaccharide, were compared with the protective activities of RNA and LPS from the parent strain, PAC 1R. The protective activity of LPS from PAC 605 was 1000 fold lower than the protective activity of LPS from PAC IR. RNA preparations of both strains induced similar percentages of survival. The protective activity of ribosomal RNA from P. aeruginosa was nonspecific since mice were also protected against a heterologous serotype of P. aeruginosa and against Escherichia coli. RNA from ribosomes of P. aeruginosa, E. coli and the non-lipopolysaccharide containing Saccharomyces cerevisiae had similar protective activities. No protection was obtained with the ribonucleic acid from the E. coliphage MS 2, It is concluded that ribosomal RNA has protective activities distinct from those of LPS.