Protective action of hepatocyte growth factor for acute liver injury caused by D-galactosamine in transgenic mice
✍ Scribed by J Okano; G Shiota; H Kawasaki
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 589 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0270-9139
No coin nor oath required. For personal study only.
✦ Synopsis
Hepatocyte growth factor (HGF) has been reported to be
Hepatocyte growth factor (HGF) is a potent mitogen for mature hepatocytes and plays a pivotal role in liver regenera-a potent mitogen for hepatocytes in vivo and in vitro. Recent reports have shown that HGF has cytoprotective actions in tion after liver injury. HGF was identified from the serum of partially hepatectomized rats, 1 rat platelets, 2 rabbit serum, 3 acute liver injury models, but its mechanisms remain to be resolved. In the present study, we investigated whether HGF the human plasma of fulminant hepatic failure, 4 and human lung fibroblasts. 5 Numerous investigations of HGF have re-could work as an anti-hepatitis agent for acute liver injury caused by D-galactosamine (D-GalN) using transgenic (TG) vealed that HGF has pleiotropic functions such as a mitogen, a morphogen, a motogen, and a tumor suppressor. [6][7][8] Re-mice expressing HGF in hepatocytes, compared with wildtype (WT) mice of their siblings. After administration of 3 g/ cently, it has been reported that HGF works as an antihepatitis agent against acute liver injuries of in vivo and in kg body weight of D-GalN, elevated serum transaminase levels and severe liver damage that were observed in WT mice were vitro models, [9][10][11][12] but its exact mechanisms remain to be clari-Abbreviations: HGF, hepatocyte growth factor; PGE2, prostaglandin E2; WT, wild type; D-GalN, D-galactosamine; TG, transgenic; ALT, alanine transaminase; AST, aspar-nated at 12-hour intervals. The male TG and WT mice were used tate transaminase; PCNA, proliferating cell nuclear antigen.
in the experiments. The age of mice in the present study ranged From the Second Department of Internal Medicine, Tottori University School of from 2 to 3 months, and the body weights of the animals were Medicine, Yonago, Japan. between 25 to 30 g. TG mice were identified by means of standard