𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Protection of 5α-dihydrotestosterone against TGF-β-induced apoptosis in FaO cells and induction of mitosis in HepG2 cells

✍ Scribed by In Kyoung Lim; Hee Jae Joo; Kyeong Sook Choi; Eisaburo Sueoka; Myung Soog Lee; Min Sook Ryu; Hirota Fujiki

Publisher
John Wiley and Sons
Year
1997
Tongue
French
Weight
329 KB
Volume
72
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Administration of TGF-b1 to both FaO and HepG 2 cells significantly induced apoptosis, particularly in FaO cells. Degradation of genomic DNA in FaO cells was rapidly induced by treatment with TGF-b1 (5 ng/ml) for only 4 hr. 5a-dihydrotestosterone (DHT, 25 nM) alone did not affect any significant changes in cell viability and in nuclei of FaO cells; however, pre-treatment with DHT protected genomic DNA degradation induced by TGF-b1 for 14 hr. Simultaneous treatment with DHT plus TGF-b1 (D 1 T) inhibited TGF-binduced apoptosis by approximately 50% in FaO cells. On the other hand, D 1 T treatment increased mitosis in actively growing HepG 2 cells. Thus, it is reasonable to conclude that DHT gives growth advantage to hepatocellular-carcinoma cells by inhibiting TGF-b-induced DNA fragmentation in FaO cells and by inducting mitosis in HepG 2 cells. Int.

📜 SIMILAR VOLUMES

Cyclic strain induces reorganization of
Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
✍ Yoshiko Yano; John Geibel; Bauer E. Sumpio 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 498 KB 👁 1 views

Cyclic strain has been shown to modulate endothelial cell (EC) morphology, proliferation, and function. We have recently reported that the focal adhesion proteins focal adhesion kinase (pp125 FAK ) and paxillin, are tyrosine phosphorylated in EC exposed to strain and these events regulate the morpho

bcl-2 and bak may play a pivotal role in
bcl-2 and bak may play a pivotal role in sodium butyrate-induced apoptosis in colonic epithelial cells; however overexpression of bcl-2 does not protect against bak-mediated apoptosis
✍ Angela Hague; G. Darío Díaz; Diane J. Hicks; Stanislaw Krajewski; John C. Reed; 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 154 KB 👁 1 views

Butyrate, a short chain fatty acid produced in the colon as a result of fermentation of dietary fibre by symbiotic bacteria, induces apoptosis in colonic tumour cell lines. Three human colonic adenoma cell lines (AA/C1, RG/C2 and BH/C1) and one carcinoma cell line (S/KS/FI) were used to determine th