Protection and Labelling of Thymidine by a Fluorescent Photolabile Group

✍ Scribed by Caroline Muller; Pascale Even; Marie-Laure Viriot;; Marie-Christiane Carré

• Book ID: 101393100
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: German
• Weight: 130 KB
• Volume: 84
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/1522-2675(20011219)84:12<3735::aid-hlca3735>3.0.co;2-a

✦ Synopsis

Dedicated to Professor Andre¬ M. Braun on the occasion of his 60th birthday A fluorescent photolabile group including coumarin and MeNPOC moieties was synthesized to protect 5'-OH terminal function of thymidine (T). Its photochemical and photophysical properties were studied, in particular the photocleavage (photodeprotection under a 365-nm irradiation) is only lowered by a factor of two by addition of the fluorophore. Fluorescence properties of the coumarin probe are not changed upon irradiation, which is satisfactory for the application required, i.e., in situ synthesis of DNA microarrays.