Protection against Plasmodium falciparum challenge induced in Aotus monkeys by liver-stage antigen-3-derived long synthetic peptides

✍ Scribed by Blanca-Liliana Perlaza; Anais Zully Valencia; Constanza Zapata; Angélica Castellanos; Jean-Pierre Sauzet; Catherine Blanc; Joe Cohen; Myriam Arévalo-Herrera; Giampietro Corradin; Sócrates Herrera; Pierre Druilhe

• Book ID: 102168808
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 132 KB
• Volume: 38
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.200738055

✦ Synopsis

Abstract

The vaccine potential of Plasmodium falciparum liver stage antigen‐3 (LSA3) was investigated in Aotus monkeys using two long synthetic peptides corresponding respectively to an N‐terminal non‐repeat peptide (NRP) and repeat 2 (R2) region of the LSA3, adjuvanted by ASO2. Both 100–222 (NRP) and 501–596 repeat peptides induced effector B‐ and T‐cell responses in terms of antigen‐driven antibodies and/or specific IFN‐γ secretion. Animals challenged with P. falciparum sporozoites were protected following immunization with either the NRP region alone or the NRP combined with the R2 repeat region, as compared with controls receiving the adjuvant alone. These results indicate that the NRP may be sufficient to induce full, sterile protection and confirm the vaccine potential of LSA3 previously demonstrated in chimpanzees and in Aotus.