𝔖 Bobbio Scriptorium
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Proteases stimulate proliferation of human fibroblasts

✍ Scribed by Pirkko Pohjanpelto

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
429 KB
Volume
91
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Incubation of primary human fibroblasts in serum‐free medium with small concentrations of thrombin, trypsin or plasmin resulted in manyfold increase in total DNA synthesis and in the number of ^3^H‐thymidine labelled cells. Rise in the frequency of mitoses indicates that the proteases stimulated also cell division. Because proteases induced only a fraction of cells to proliferate increase in the total number of cells remained moderate. Calf, horse and rabbit serum inhibited the growth stimulating effect of trypsin but chicken, dog and monkey serum were permissive. Specific inhibitors of proteases prevented the stimulation of cell proliferation suggesting strongly that proteases act in virtue of their enzymatic activity.

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