Production of erythropoietin by BHK cell
Production of erythropoietin by BHK cells growing on the microcarriers trapped in alginate gel beads
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Yoshihito Shirai; Kenji Hashimoto; Hiroyuki Kawahara; Ryuzo Sasaki; Kiyotaka Hit
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Article
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1989
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Springer
🌐
English
⚖ 359 KB
Baby hamster kidney (BHK) cells engineered to produce recombinant human erythropoietin (EPO) were cultured at high density on microcarriers entrapped by calcium alginate gel particles. In this system, the BHK cells proliferated not only on the microcarriers but also in vacant spaces in the alginate