Protease inhibitors from Streptomyces violascens

✍ Scribed by Torstensson, N. T. L. ;J�nsson, A. G.

• Book ID: 104769971
• Publisher: Springer-Verlag
• Year: 1972
• Weight: 798 KB
• Volume: 83
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00425046

✦ Synopsis

A double-layer agar method was developed for screening microorganisms producing protease inhibitors. After colonies were grouch on skim-milk agar, an agar containing sterile protease was poured onto the bottom layer. After incubation the skim-milk agar turned transparent except around colonies producing protease inhibitors. The frequency of such organisms in samples of soil, manure, and hay was studied. 27 of more than 10000 colonies showed a significant proteaseinhibiting capacity. One organism with a high trypsin-inhibiting capacity was identified as Streptomyces violascens (Preobrazhenskaya and Sveshnikova) Pridham et al. and selected for further studies.