Prostatic ductal system in rats: Tissue-specific expression and regional variation in stromal distribution of transforming growth factor-β1
✍ Scribed by Nemeth, Jeffrey A.; Sensibar, Julia A.; White, Roxanne R.; Zelner, David J.; Kim, Isaac Y.; Lee, Chung
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 275 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0270-4137
No coin nor oath required. For personal study only.
✦ Synopsis
Background:
Regional variations in stromal-epithelial interactions, mediated through soluble growth factors, may be responsible for differences in epithelial growth and death observed between regions of the rat prostatic ductal system. since transforming growth factor-beta 1 (tgf-beta 1) can induce prostatic epithelial cell death in vitro and in vivo, we examined the localization and production of tgf-beta 1 with respect to the functional regions of the rat prostatic ductal system.
Methods:
The distribution of tgf-beta 1 in the rat ventral prostate was examined by immunohistochemistry. cell type-specific expression of tgf-beta 1 was determined using rt-pcr analysis of prostate epithelial and stromal cell fractions separated by percoll gradient centrifugation.
Results:
Immunohistochemical staining of normal prostate revealed regional variations in stromal tgf-beta 1 protein, which was most abundant in the stroma surrounding the degenerative proximal ducts. tgf-beta 1 staining was also tightly associated with the prostatic smooth muscle. results of rt-pcr experiments confirmed the major source of tgf-beta 1 mrna in normal rat prostate to be the stroma, with lesser expression by the epithelium.
Conclusions:
Stromal tgf-beta 1 was associated with cell death in the adjacent epithelial cell compartment in the prostatic ductal system, and alpha-smooth muscle actin-positive stromal cells may play a negative growth-regulatory role in the rat ventral prostate through production of tgf-beta 1.