Prostaglandin E2 stimulates osteoclast-like cell formation and bone-resorbing activity via osteoblasts: Role of cAMP-dependent protein kinase
✍ Scribed by Hiroshi Kaji; Toshitsugu Sugimoto; Masanori Kanatani; Masaaki Fukase; Masayoshi Kumegawa; Kazuo Chihara
- Publisher
- American Society for Bone and Mineral Research
- Year
- 2009
- Tongue
- English
- Weight
- 944 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0884-0431
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✦ Synopsis
Prostaglandin E, (PGE,) is an important local regulator in bone. The present study was performed to investigate the effect of PGE, on osteoclast-like cell formation and bone-resorbing activity of mature osteoclasts in the presence or absence of osteoblasts. PGE, ( M) significantly stimulated osteoclast-like cell formation in osteoblast-containing mouse bone cell cultures, although it did not affect osteoclast-like cell formation from hemopoietic blast cells supported by granulocyte-macrophage colony-stimulating factor in osteoblast-free mouse spleen cell cultures. The conditioned medium from osteoblastic UMR-106 cells pretreated with PGE, (lo-' and M) significantly stimulated osteoclast-like cell formation from hemopoietic blast cells. PGE, also significantly stimulated the bone-resorbing activity of mature osteoclasts in osteoblast-containing mouse bone cell cultures. In contrast, PGE, significantly inhibited the bone-resorbing activity and osteopontin mRNA expression in isolated rabbit osteoclasts. Rp-CAMPS, a direct protein kinase (PKA) antagonist, significantly inhibited PGE,-stimulated osteoclast-like cell formation and the bone-resorbing activity of mature osteoclasts, although protein kinase C inhibitors, dantrolene (an inhibitor of calcium release from the intracellular calcium pool) and voltage-dependent calcium channel blockers did not affect PGE,-stimulated osteoclast-like cell formation. In conclusion, PGE, stimulated osteoclast-like cell formation and bone-resorbing activity in mouse bone cell cultures presumably through osteoblasts. The activation of PKA is linked to PGE,-stimulated osteoclast-like cell formation and bone-resorbing activity. (J Bone Miner Res 1996;11:62-71) to 'Third Division,