Prostaglandin biosynthesis by midgut tissue isolated from the tobacco hornworm, Manduca sexta
✍ Scribed by K. Büyükgüzel; H. Tunaz; S.M. Putnam; D. Stanley
- Book ID
- 117673073
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 177 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0965-1748
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We report on secretion of phospholipase A 2 (PLA 2 ) by in vitro preparations of midguts isolated from tobacco hornworms, Manduca sexta. This enzyme is responsible for hydrolysis of fatty acids from the sn-2 position of phospholipids, a necessary step in fatty acid absorption. The in vitro midgut pr
Chloride-stimulated K+ secretion by Manduca sexta midgut (5th-instar larvae) was measured as K(+)-carried short-circuit current of the tissue mounted in an Ussing chamber. "Microscopic" parameters, such as single-channel current and channel density for the rate-determining passive transport step acr