Prospects and implications of using chromatin insulators in gene therapy and transgenesis

Analysis and use of endogenous nuclease

Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy

✍ G. A. Monteiro; G. N. M. Ferreira; J. M. S. Cabral; D. M. F. Prazeres 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB 👁 1 views

Two important issues in the downstream processing of plasmids for gene therapy are the stability of plasmids in the process streams, and the presence of contaminating host RNA. Results with a 4.8-kb plasmid harbored in a non-nuclease-deficient strain of Escherichia coli show that, in spite of the ha