Prosencephalic neural folds give rise to neural crest cells in the Australian lungfish, Neoceratodus forsteri

✍ Scribed by Martin Kundrát; Jean M. P. Joss; Lennart Olsson

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 839 KB
• Volume: 312B
• Category: Article
• ISSN: 1552-5007
• DOI: 10.1002/jez.b.21248

✦ Synopsis

Abstract

Here we present a fate map of the prosencephalic neural fold (PNF) for the Australian lungfish. The experimental procedures were carried out on lungfish embryos at Kemp's stage 24 using three different approaches. First, either medial PNF (MPNF) or lateral PNF (LPNF) were ablated and the embryos cultured until they reached Kemp's stage 42 and 44. Ablation of the LPNF provided phenotypes with arrested development of the eye, reduction of periocular pigmentation, frontonasal deformity, and a slightly reduced olfactory organ, whereas the MPNF‐ablated phenotypes resulted in arrested development of the cornea and frontonasal deformity. Second, we labeled the mid‐axial level of the PNF with vital DiI and traced the migration of labeled cells following culture to Kemp's stage 33. Labeled PNF‐derived cells populated a basal layer of the olfactory placode, migrated into the frontonasal region, the antero‐dorsal periocular quadrant, and also terminated at positions where the forebrain meninges form at later stages. Third, we examined HNK‐1 immunoreactivity in the forebrain‐related region. We conclude that in the Australian lungfish: (1) LPNF‐derived neuroepithelium gives rise to the basal layer and contributes to the apical layer of the olfactory placode; (2) PNF‐derived NC cells appear to give rise to meningeal, periocular, and frontonasal ectomesenchyme and likely infiltrate the olfactory placode as developmental precusors of the terminal nerve; (3) HNK‐1 epitope is temporarily expressed in cells of the neural tube, NC cells, and neurogenic placodal cells. Our experiments have provided the first evidence for a premandibular NC stream (sensu Kundrát, 2008) in a fish. J. Exp. Zool. (Mol. Dev. Evol.) 312B:83–94, 2009. © 2008 Wiley‐Liss, Inc.