Prophage induction inEscherichia coli K12 cells deficient in DNA polymerase I
β Scribed by Blanco, Manuel ;Pomes, Lina
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 465 KB
- Volume
- 154
- Category
- Article
- ISSN
- 0026-8925
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The effect of the cellular level of RecA protein on the ability of E. coli K12 bacteria to (i) survive UV-irradiation (ii) promote UV-reactivation of UV-damaged phage lambda (iii) induce prophage lambda was determined in bacterial mutants with discrete increasing levels of RecA protein. The various
Using phiX1974 replicative form (RF) DNA as an in vivo probe, we have investigated the coordinated action of the 5' leads to 3' exonuclease and polymerase activities of DNA polymerase I in order to understand better its physiological role. We constructed double mutants containing the rep mutation (t