Properties of the transducing phage Ml of Rhizobium meliloti
✍ Scribed by Wanda Malek
- Book ID
- 102393890
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 421 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
A temperate phage MI, infective towards some R . meliloii strains, was isolated from soil taken from local fields. Its morphology, host range, transducing ability and the locations of restriction endonuclease cleavage sites on the genomes were determined. Phage MI was chssified as belonging to B BRADLEY'S group bacteriophages because of the hexagonal outlines of its head and its long, flexible and non-contractible tail. The molecular weight of phage DNA was estimated to be 54.1 & I kilobases (kb) and it corresponded to the value expected for capsid size -70 nm. Phage M1 carries out general transduction and forms stable lysogens with the R. melilori strain L5.30.
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Restriction cleavage maps for enzymes EcoRI, BamHI, PstI, PvulI, XbaI and EcoRV of Rhizobium meliloti temperate phage 16-3 have been established. Together with the earlier maps (HindlII, KpnI, HpaI, BgllI) 98 restriction sites, 'evenly' distributed, have been mapped along the phage genome, including
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