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Properties of the cyclic AMP-dependent protein kinases in mouse mastocytoma cells

✍ Scribed by John Smart; Ray Ralph

Publisher
Springer
Year
1983
Tongue
English
Weight
473 KB
Volume
50
Category
Article
ISSN
0300-8177

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✦ Synopsis

The properties of type I and type II protein kinases in PY815 mouse mastocytoma cells were shown to change following growth inhibition by prostaglandin E1 and 3-isobutyl-1-methylxanthine. These changes included a large reduction in type I protein kinase consistent with a role for this isoenzyme as a positive effector of growth, a decrease in free cyclic AMP binding protein and an increase in type II protein kinase. Some properties of the fully activated isoenzymes are presented that may be important in determining their activity in vivo.

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