Properties of mitotic cells prepared by mechanically shaking monolayer cultures of chinese hamster cells

✍ Scribed by R. A. Tobey; E. C. Anderson; D. F. Petersen

• Book ID: 102308331
• Publisher: John Wiley and Sons
• Year: 1967
• Tongue: English
• Weight: 450 KB
• Volume: 70
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040700109

✦ Synopsis

A technique has been developed for the selective detachment of mitotic cells from monolayer cultures of Chinese hamster cells with a simple reciprocating shaking machine. Cultures prepared by the shake treatment and placed in spinner flasks are routinely obtained, in which the mitotic fraction drops from 0.95-0.05 in 19 minutes. Some properties of mitotic cells prepared by this technique are described, along with a simple procedure for producing large quantities of mitotic cells. Cells chilled immediately after collection from a series of shake treatments complete mitosis synchronously upon subsequent resuspension in warm medium.