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Properties of hybrid plasmids, consisting of parts of the mini-R1 factorRsc11 andColE1

โœ Scribed by Boidol, W. ;Siewert, G. ;Lindenmaier, W. ;Luibrand, G. ;Goebel, W.

Publisher
Springer
Year
1977
Tongue
English
Weight
641 KB
Volume
152
Category
Article
ISSN
0026-8925

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The in vitro synthesis of the mini R1-factor, Rsc11, was achieved using a soluble Escherichia coli cell-extract system. Triton X-100 lysates of the K12 strain 1101 (Rsc11) fractionated by Sephadex G25 chromatography supported the incorporation of labeled deoxyribonucleotides into covalently-closed c