Properties of a thermostable and solvent stable extracellular lipase from a Pseudomonas sp. AG-8

✍ Scribed by Anil K. Sharma; Ram P. Tiwari; Gurinder S. Hoondal

• Book ID: 101383319
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 75 KB
• Volume: 41
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/1521-4028(200112)41:6<363::aid-jobm363>3.0.co;2-c

✦ Synopsis

An extracellular lipase isolated from Pseudomonas sp. AG-8, had an optimal activity at 45 °C and pH 8.0 -8.5. It retained more than 80% of its initial activity after keeping for 1 h at 65 °C. The enzyme was stable in 5 M NaCl and 6 M urea. Triton X-100 increased the lipase activity by 2.4 fold. Ca 2+ ions activated the enzyme, while Zn 2+ , Fe 2+ , Fe 3+ strongly inhibited its activity. Ethanol, methanol and acetone at 20% (v/v) enhanced the lipase activity by 2.9, 3.6 and 4.5 fold respectively. Dimethylsulphoxide at 90% (v/v) enhanced the enzyme activity up to 5.7 fold.