Properties of a Leu-Phe-Cleaving Endopeptidase Activity Putatively Involved in β-Endorphin Metabolism in Rat Brain

Quantitation of the endopeptidase activi

Quantitation of the endopeptidase activity generating γ-endorphin from β-endorphin in rat brain synaptic membranes by a radiometric assay

✍ Jos L.M. Lebouille; J.Peter H. Burbach; E.Ronald de Kloet 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 768 KB

gamma-Endorphin is a naturally occurring biologically active peptide that is produced by an endopeptidase activity cleaving its precursor beta-endorphin. This enzyme was termed gamma-endorphin generating enzyme (gamma-EGE). In order to quantitate gamma-EGE activity by means of a simple and sensitive