Properties and kinetics of glucan phosphorylase of the amylose-forming yeast Cryptococcus laurentii
β Scribed by M. S. Foda; Prof. Dr. H. J. Phaff
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 713 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0233-111X
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β¦ Synopsis
Properties and kinetics of glucan phosphorylase of the amylose-forming yeast Cryptococcus laurentii
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An acid phosphatase from derepressed cells of the yeast form of Yarrowia lipolyrica was purified 176-fold by ammonium sulfate precipitation, chromatography on DEAE-Sephacel and gel filtration in Biogel A 5-M. The enzyme is a glycoprotein with a sugar content of 60%. The MICHAELIS constant of the enz