Water-resistant films were prepared by coating a castor oil-based polyurethane-chitosan (PU-CH), in which grafted interpenetrating polymer networks (IPNs) were produced, on a regenerated cellulose (RC) film. The tensile strengths of the coated films cured at 90ЊC for 5 min reached 853 kg cm 02 (dry state) and 503 kg cm 02 (wet state) and were obviously higher than those of the films of uncoated and coated with PU coating. Moreover, the coated films have excellent water resistivity, low vapor permeability, and good size stability, and their optical transmittance is even better than that of the RC film in the range of 400-800 nm. The interfacial structure of the coated films was investigated by using spectroscopy infrared, ultraviolet spectroscopy, transmission electron microscopy, and electron probe microanalysis. It was shown that the strong interfacial bonding with chemical and hydrogen bonds between the RC film and the coating exists. The PU prepolymer in the IPN coating penetrated through the interface into the RC film and partly crosslinked with the cellulose, forming a semi-IPNs. The chitosan in the PU-CH coating plays an important role not only in accelerating the cure of the coating but also in improving the mechanical properties and biodegradability of the coated film.