Properties and growth mechanism of the ordered aggregation of a model RNA by the HIV-1 nucleocapsid protein: An electron microscopy investigation
✍ Scribed by Eric Le Cam; Dominique Coulaud; Etienne Delain; Patrice Petitjean; Bernard P. Roques; Dominique Gérard; Elena Stoylova; Constance Vuilleumier; Stoyl P. Stoylov; Yves Mély
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1998
- Tongue
- English
- Weight
- 420 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
NCp7, the nucleocapsid protein of the human immunodeficiency virus type 1, induces an ordered aggregation of RNAs, a mechanism that is thought to be involved in the NCp7-induced promotion of nucleic acid annealing. To further investigate this aggregation, the morphology and the properties of the NCp7-induced aggregates of the model RNA homoribopolymer, polyA, were investigated by electron microscopy in various conditions. In almost all the tested conditions, the aggregates were spherical and consisted of a central dense core surrounded by a less dense halo made of NCp7-covered polyA molecules. The formation of