Properties and cellular localization of a luciferin binding protein in the bioluminescence reaction ofGonyaulax polyedra
✍ Scribed by Morse, D. ;Fritz, L. ;Pappenheimer, A. M. ;Hastings, J. W.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 305 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0884-3996
No coin nor oath required. For personal study only.
✦ Synopsis
A luciferin binding protein LBP involved in the bioluminescence reaction of Gonyaulax po/yedra was purified and used for antibody production. Luciferin bound to LBP is fluorescent and can be used as a marker in living cells, allowing the localization of LBP in cortical organelles to be visualized. In cell sections, the same peripheral localization was observed using anti-LBP and immunofluorescence microscopy. The amount of LBP is ten-fold greater from cells from in night phase compared to those from in day phase, as determined both by immunoblots of cell extracts, and in v i m fluorescence. These changes correlate with the circadian changes in bioluminescence of living cells.