Properties and application of inulinase obtained by fermentation of costus (Saussurea lappa) root powder withAspergillus niger
✍ Scribed by Viswanathan, Poorna ;Kulkarni, P. R.
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 393 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0027-769X
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✦ Synopsis
Abstract
Acetone precipitated extracellular inulinase from Aspergillus niger van Tieghem UV 11 grown on costus (Kuth; Saussurea lappa) root powder/corn steep liquor medium was found to have a pH optimum of 5.5, temperature optimum of 50 °C and stability at 50 °C for 60 min. Substrate affinity was in the order of inulin > sucrose > raffinose. At 0.001 M concentrations, Mg^2+^ and Ca^2+^ were weak activators of inulinase while Fe^3+^, Cu^2+^, Zn^2+^, Co^2+^ and Mn^2+^ were found to be inhibitors of inulinase. Hydrolysis of inulin extracted and purified from costus root powder and standard inulin with inulinase under similar conditions yielded 70% fructose and 68% fructose, respectively.