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Propagation of fluorescent viruses in growing plaques

✍ Scribed by Luis J. Alvarez; Philippe Thomen; Tatyana Makushok; Didier Chatenay

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 283 KB
Volume: 96
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.21110

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✦ Synopsis

Abstract

To study virus propagation, we have developed a method by which the propagation of the Lambda bacteriophage can be observed and quantified. This is done by creating a fusion protein of the capsid protein gpD and the enhanced yellow fluorescent protein (EYFP). We show that this fusion allows capsid formation and that the modified viruses propagate on a surface covered with host bacteria thus forming fluorescent plaques. The intensity of fluorescence in a growing plaque determines the distribution of phages. This provides a new tool to study the propagation of infection at the microscopic level. Biotechnol. Bioeng. 2007;96: 615–621. © 2006 Wiley Periodicals, Inc.

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