Promoter methylation and transgene copy numbers predict unstable protein production in recombinant chinese hamster ovary cell lines

Osmoprotective effect of glycine betaine

Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines

✍ Joon Soo Ryu; Tae Kyung Kim; Joo Young Chung; Gyun Min Lee 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 166 KB 👁 2 views

When three recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B22-4, CS13-1.00\*, and CS13-0.02\*, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. However, due to a simultaneous suppression o